Dr. Bharathwaj Sathyamoorthy

Biomolecular NMR Spectroscopy

Academic Details

Postdoctoral Research Fellow,Duke University, Durham, NC, USA, Advisor: Prof. Hashim M. Al-Hashimi,Jan 2014 – Nov 2015.

Postdoctoral Research Fellow, University of Michgan, Ann Arbor, MI, USA Advisor: Prof. Hashim M. Al-Hashimi, Apr – Dec 2013.

Ph. D. from State University of New York at Buffalo, Buffalo, NY, USA Advisor: Prof. Thomas Szyperski 2013

M. Sc. from Indian Institute of Technology Madras, Chennai, Tamil Nadu, India ,2007.

B.Sc. from Ramakrishna Mission Vivekananda College, Chennai, Tamil Nadu, India, 2005.

Profile Details

    Traditionally biomolecular function has been primarily associated with their structure. Complementing X-ray crystallography is solution state Nuclear Magnetic Resonance (NMR) spectroscopy that can characterize biomolecular structure at atomic resolution. In addition, NMR spectroscopy also has the advantage of visualizing dynamics aiding in understanding molecular plasticity that has been recently recognized to contribute towards diverse functions, such as folding, aggregation, recognition, catalysis, replication, misincorporation, damage induction/accommodation/repair, etc.

    My lab would be involved in applying conventional and advanced NMR methodology to characterize structure and dynamics of various biomolecules. Thermodynamic and kinetic characterization would be performed using basic physical chemistry principles for vital biological problems. The results would be interpreted to describe the function of the molecule and shall be applied to study various systems, such as DNA misincorporation/ mismatches and damage, kinetics of RNA refolding, etc. In addition to existing methods, new NMR methods shall be developed, implemented and applied to further our biophysical understanding of proteins and nucleic acids. Long-term goals would be extending the methodology to study protein-nucleic acid complexes.

    • Spatially Selective Heteronuclear Multiple-Quantum Coherence (HMQC) Spectroscopy for Biomolecular NMR Studies. Bharathwaj Sathyamoorthy, David M. Parish, Gaetano T. Montelione, Rong Xiao, Thomas Szyperski. ChemPhysChem, 2014, 15(9), 1872-1879. DOI: 10.1002/cphc.201301232.

    • Computational de novo Design of a Four-Helix Bundle Protein - DND_4HB.Grant S. Murphy,Bharathwaj Sathyamoorthy,BryanS. Der,MischaC.Machius,SuryaV.Pulavarti,GaetanoT. Montelione,ThomasSzyperski,Brian Kuhlman. Protein Sci, 2014, Online. DOI: 10.1002/pro.2577.

    • Protein Conformational Space Populated in Solution Probed with Aromatic Residual Dipolar 13C -1H Couplings. Bharathwaj Sathyamoorthy, Kiran K. Singarapu, Angel E. Garcia, ThomasSzyperski.ChemBioChem,2013, 14(6), 684-688. DOI:10.1002/cbic.201300016.

    • Development and Application of Aromatic SOFAST-HMQC NMR Experiments for Nucleic Acids. Bharathwaj Sathyamoorthy, Janghyun Lee, Isaac Kimsey, Hashim Al-Hashimi. J Biomol NMR, 2014, 60 (2-3), 77-83. DOI: 10.1007/s10858-014-9856-9.

    • Visualizing Transient Watson-Crick Like Mispairs in DNA and RNA Duplexes. Isaac J. Kimsey, Katja Petzold, Bharathwaj Sathyamoorthy, Zachary W. Stein and Hashim M. Al-Hashimi. Nature, 2015, 519, 315-320. DOI: 10.1038/nature14227.