Profile || Department of Chemistry

Bharathwaj Sathyamoorthy

Biomolecular NMR Spectroscopy
Assistant Professor
Phone: +91 755 269 1323, 669 1323
bharathwaj@iiserb.ac.in

Postdoctoral Research Fellow,Duke University, Durham, NC, USA, Advisor: Prof. Hashim M. Al-Hashimi,Jan 2014 – Nov 2015.

Postdoctoral Research Fellow, University of Michgan, Ann Arbor, MI, USA Advisor: Prof. Hashim M. Al-Hashimi, Apr – Dec 2013.

Ph. D. from State University of New York at Buffalo, Buffalo, NY, USA Advisor: Prof. Thomas Szyperski 2013

M. Sc. from Indian Institute of Technology Madras, Chennai, Tamil Nadu, India ,2007.

B.Sc. from Ramakrishna Mission Vivekananda College, Chennai, Tamil Nadu, India, 2005.

Traditionally biomolecular function has been primarily associated with their structure. Complementing X-ray crystallography is solution state Nuclear Magnetic Resonance (NMR) spectroscopy that can characterize biomolecular structure at atomic resolution. In addition, NMR spectroscopy also has the advantage of visualizing dynamics aiding in understanding molecular plasticity that has been recently recognized to contribute towards diverse functions, such as folding, aggregation, recognition, catalysis, replication, misincorporation, damage induction/accommodation/repair, etc.

My lab would be involved in applying conventional and advanced NMR methodology to characterize structure and dynamics of various biomolecules. Thermodynamic and kinetic characterization would be performed using basic physical chemistry principles for vital biological problems. The results would be interpreted to describe the function of the molecule and shall be applied to study various systems, such as DNA misincorporation/ mismatches and damage, kinetics of RNA refolding, etc. In addition to existing methods, new NMR methods shall be developed, implemented and applied to further our biophysical understanding of proteins and nucleic acids. Long-term goals would be extending the methodology to study protein-nucleic acid complexes.

#	Authors	Title	Journals	Year
1	Arjun Rana Rajesh Kumar Reddy Sannapureddi Manjula Jaisal Bharathwaj Sathyamoorthy	5-Formylcytosine weakens the G–C pair and imparts local conformational fluctuations to DNA duplexes	Physical Chemistry Chemical Physics (PCCP)	2023
2	Bharathwaj Sathyamoorthy Rajesh Kumar Reddy Sannapureddi Deepraj Negi Pooja Singh	Conformational Characterization of Duplex DNA with Solution-state NMR Spectroscopy	Journal of Magnetic Resonance Open	2022
3	Satendra Kumar Rajesh Kumar Reddy Sannapureddi Chaitra S. Todankar Annyesha Biswas Bharathwaj Sathyamoorthy Pradeepkumar PI	Bisindolylmaleimide Ligands Stabilize c-MYC G-Quadruplex DNA Structure and Downregulate Gene Expression	Biochemistry	2022
4	Mahdad Noursadeghi Bharathwaj Sathyamoorthy Sreevidhya Tarakkad Krishnaji Helena J Tutill Akhilesh Kumar Ranjeet Singh Mahla Himanshu Kumar Amit Kumar Pandey Judith Breuer	NIX-mediated mitophagy regulate metabolic reprogramming in phagocytic cells during mycobacterial infection	Tuberculosis	2021
5	Prashant Kumar Bharathwaj Sathyamoorthy Abhishek Cukkemane	A metabolomics footprint approach to understanding the benefits of synbiotics in functional foods and dietary therapeutics for health, communicable and non-communicable diseases	Food Research International	2020
6	Bharathwaj Sathyamoorthy Anoop Kumar Gautam Manish Kumar Mohanty Rajesh Kumar Reddy Sannapureddi	Characterization of DNA G-quadruplex Topologies with NMR Chemical Shifts	The Journal of Physical Chemistry Letters	2020
7	Phoebe A Rice David A Case Ying Zhang Pigli Yi Xue Yu Xu Allison Stelling Bharathwaj Sathyamoorthy Huiqing Zhou Hashim M Al-Hashimi	Characterizing Watson-Crick versus Hoogsteen Base Pairing in a DNA-Protein Complex Using Nuclear Magnetic Resonance and Site-Specifically 13C- and 15N-Labeled DNA	Biochemistry	2019
8	Bharathwaj Sathyamoorthy Chia-Chieh Chu Yi Xue Anisha Shakya Walter J Zahurancik Eric S Szymanski Isaac J Kimsey Zucai Suo Hashim M Al-Hashimi	Dynamic basis for dGâ€¢dT misincorporation via tautomerization and ionization	Nature ( Through e-Shodh Sindhu)	2018
9	David A Case Bharathwaj Sathyamoorthy Atul Rangadurai Mary C Clay Honglue Shi Hashim M Al-Hashimi	Atomic structures of excited state Aâ€“T Hoogsteen base pairs in duplex DNA by combining NMR relaxation dispersion, mutagenesis, and chemical shift calculations	Eur. J. Inorg. Chem.	2018
10	Bharathwaj Sathyamoorthy Aman D Kansal Megan L Kelly Dawn K Merriman Atul Rangadurai Janghyun Lee Laura R Ganser Hashim M Al-Hashimi	High-performance virtual screening by targeting a high-resolution RNA dynamic ensemble	Nature Structural and Molecular Biology	2018
11	Atul Rangadurai Yi Xue Huiqing Zhou Honglue Shi Bharathwaj Sathyamoorthy Dawn Kellogg Merriman Hashim M Al-Hashimi	Insights into Watson-Crick/Hoogsteen breathing dynamics and damage repair from the solution structure and dynamic ensemble of DNA duplexes containing m1A	Nucleic Acids Research	2017
12	Bharathwaj Sathyamoorthy Janghyun Lee Christopher S Eubanks Gary J Kapral Laura R Ganser Neeraj N Patwardhan Hashim M Al-Hashimi Amanda E Hargrove	Amiloride as a new RNA-binding scaffold with activity against HIV-1 TAR	Eur. J. Inorg. Chem.	2017