Bharathwaj Sathyamoorthy

Biomolecular NMR Spectroscopy

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Academic Details

Postdoctoral Research Fellow,Duke University, Durham, NC, USA, Advisor: Prof. Hashim M. Al-Hashimi,Jan 2014 – Nov 2015.

Postdoctoral Research Fellow, University of Michgan, Ann Arbor, MI, USA Advisor: Prof. Hashim M. Al-Hashimi, Apr – Dec 2013.

Ph. D. from State University of New York at Buffalo, Buffalo, NY, USA Advisor: Prof. Thomas Szyperski 2013

M. Sc. from Indian Institute of Technology Madras, Chennai, Tamil Nadu, India ,2007.

B.Sc. from Ramakrishna Mission Vivekananda College, Chennai, Tamil Nadu, India, 2005.

Profile Details

    Traditionally biomolecular function has been primarily associated with their structure. Complementing X-ray crystallography is solution state Nuclear Magnetic Resonance (NMR) spectroscopy that can characterize biomolecular structure at atomic resolution. In addition, NMR spectroscopy also has the advantage of visualizing dynamics aiding in understanding molecular plasticity that has been recently recognized to contribute towards diverse functions, such as folding, aggregation, recognition, catalysis, replication, misincorporation, damage induction/accommodation/repair, etc.

    My lab would be involved in applying conventional and advanced NMR methodology to characterize structure and dynamics of various biomolecules. Thermodynamic and kinetic characterization would be performed using basic physical chemistry principles for vital biological problems. The results would be interpreted to describe the function of the molecule and shall be applied to study various systems, such as DNA misincorporation/ mismatches and damage, kinetics of RNA refolding, etc. In addition to existing methods, new NMR methods shall be developed, implemented and applied to further our biophysical understanding of proteins and nucleic acids. Long-term goals would be extending the methodology to study protein-nucleic acid complexes.

Authors Title of Article Journal Year
Mahdad Noursadeghi
Bharathwaj Sathyamoorthy
Sreevidhya Tarakkad Krishnaji
Helena J Tutill
Akhilesh Kumar
Ranjeet Singh Mahla
Himanshu Kumar
Amit Kumar Pandey
Judith Breuer
NIX-mediated mitophagy regulate metabolic reprogramming in phagocytic cells during mycobacterial infection
Details
Tuberculosis 2021
Prashant Kumar
Bharathwaj Sathyamoorthy
Abhishek Cukkemane
A metabolomics footprint approach to understanding the benefits of synbiotics in functional foods and dietary therapeutics for health, communicable and non-communicable diseases
Details
Food Research International 2020
Bharathwaj Sathyamoorthy
Anoop Kumar Gautam
Manish Kumar Mohanty
Rajesh Kumar Reddy Sannapureddi
Characterization of DNA G-quadruplex Topologies with NMR Chemical Shifts
Details
The Journal of Physical Chemistry Letters 2020
Phoebe A Rice
David A Case
Ying Zhang Pigli
Yi Xue
Yu Xu
Allison Stelling
Bharathwaj Sathyamoorthy
Huiqing Zhou
Hashim M Al-Hashimi
Characterizing Watson-Crick versus Hoogsteen Base Pairing in a DNA-Protein Complex Using Nuclear Magnetic Resonance and Site-Specifically 13C- and 15N-Labeled DNA
Details
Biochemistry 2019
Bharathwaj Sathyamoorthy
Chia-Chieh Chu
Yi Xue
Anisha Shakya
Walter J Zahurancik
Eric S Szymanski
Isaac J Kimsey
Zucai Suo
Hashim M Al-Hashimi
Dynamic basis for dG•dT misincorporation via tautomerization and ionization
Details
Nature ( Through e-Shodh Sindhu) 2018
David A Case
Bharathwaj Sathyamoorthy
Atul Rangadurai
Mary C Clay
Honglue Shi
Hashim M Al-Hashimi
Atomic structures of excited state A–T Hoogsteen base pairs in duplex DNA by combining NMR relaxation dispersion, mutagenesis, and chemical shift calculations
Details
Eur. J. Inorg. Chem. 2018
Bharathwaj Sathyamoorthy
Aman D Kansal
Megan L Kelly
Dawn K Merriman
Atul Rangadurai
Janghyun Lee
Laura R Ganser
Hashim M Al-Hashimi
High-performance virtual screening by targeting a high-resolution RNA dynamic ensemble
Details
Nature Structural and Molecular Biology 2018
Atul Rangadurai
Yi Xue
Huiqing Zhou
Honglue Shi
Bharathwaj Sathyamoorthy
Dawn Kellogg Merriman
Hashim M Al-Hashimi
Insights into Watson-Crick/Hoogsteen breathing dynamics and damage repair from the solution structure and dynamic ensemble of DNA duplexes containing m1A
Details
Nucleic Acids Research 2017
Bharathwaj Sathyamoorthy
Janghyun Lee
Christopher S Eubanks
Gary J Kapral
Laura R Ganser
Neeraj N Patwardhan
Hashim M Al-Hashimi
Amanda E Hargrove
Amiloride as a new RNA-binding scaffold with activity against HIV-1 TAR
Details
Eur. J. Inorg. Chem. 2017